Avoiding and controlling double transformation artifacts

Avoiding and controlling double transformation artifacts.

This article describes a set of standard control experiments for the authentication of new protein variants isolated through library selection and site-directed mutagenesis. These controls are specifically designed to rule out artifacts derived from 'double transformants' -- i.e. cells transformed with, or infected by, two different plasmids simultaneously. These seem to have been the source of...

Recognizing and avoiding artifacts in AFM imaging.

Self-Avoiding Walks and the Fisher Transformation

The Fisher transformation acts on cubic graphs by replacing each vertex by a triangle. We explore the action of the Fisher transformation on the set of self-avoiding walks of a cubic graph. Iteration of the transformation yields a sequence of graphs with common critical exponents, and with connective constants converging geometrically to the golden mean. We consider the application of the Fishe...

compactifications and representations of transformation semigroups

this thesis deals essentially (but not from all aspects) with the extension of the notion of semigroup compactification and the construction of a general theory of semitopological nonaffine (affine) transformation semigroup compactifications. it determines those compactification which are universal with respect to some algebric or topological properties. as an application of the theory, it is i...

Avoiding electromagnetic artifacts when recording auditory steady-state responses.

Electromagnetic artifacts can occur when recording multiple auditory steady-state responses evoked by sinusoidally amplitude modulated (SAM) stimuli. High-intensity air-conducted stimuli evoked responses even when hearing was prevented by masking. Additionally, high-intensity bone-conducted stimuli evoked responses that were completely different from those evoked by air-conducted stimuli of sim...